Comparative Proteomics Analysis Reveals That Lignin Biosynthesis Contributes to Brassinosteroid-Mediated Response to Phytophthora sojae in Soybeans.
Identifieur interne : 000269 ( Main/Exploration ); précédent : 000268; suivant : 000270Comparative Proteomics Analysis Reveals That Lignin Biosynthesis Contributes to Brassinosteroid-Mediated Response to Phytophthora sojae in Soybeans.
Auteurs : Xinfang Wang [République populaire de Chine] ; Zisu Zhao [République populaire de Chine] ; Na Guo [République populaire de Chine] ; Haitang Wang [République populaire de Chine] ; Jinming Zhao [République populaire de Chine] ; Han Xing [République populaire de Chine]Source :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2020.
Abstract
Brassinosteroids (BRs) are a group of steroid plant hormones regulating normal growth, development, and stress response in plants. However, the mechanisms by which BRs interfere with the resistance of soybean to Phytophthora sojae (P. sojae) remain largely unknown. The present study analyzed the role of BRs in soybean response against P. sojae by comparative proteomic approaches. A total of 52,381 peptides were obtained by trypsin digestion of 9,680 proteins, among which 6,640 proteins were quantified, and 402 proteins were identified as differentially expressed proteins (DEPs). Further analysis revealed that DEPs were significantly involved in the lignin biosynthesis pathway. The expression of the majority of key enzymes involved in lignin biosynthesis was upregulated by BR-pretreatment and P. sojae infection, and lignin accumulation was faster in BR-pretreated soybeans than in untreated controls. Additionally, accumulation of lignin was consistent with these enzyme expressions levels and resistance phenotype. These findings advance the understanding of the role of BRs in the interaction between soybeans and P. sojae.
DOI: 10.1021/acs.jafc.0c00848
PubMed: 32302119
Affiliations:
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and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Na" sort="Guo, Na" uniqKey="Guo N" first="Na" last="Guo">Na Guo</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Haitang" sort="Wang, Haitang" uniqKey="Wang H" first="Haitang" last="Wang">Haitang Wang</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Jinming" sort="Zhao, Jinming" uniqKey="Zhao J" first="Jinming" last="Zhao">Jinming Zhao</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Xing, Han" sort="Xing, Han" uniqKey="Xing H" first="Han" last="Xing">Han Xing</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Brassinosteroids (BRs) are a group of steroid plant hormones regulating normal growth, development, and stress response in plants. However, the mechanisms by which BRs interfere with the resistance of soybean to <i>Phytophthora sojae</i> (<i>P. sojae</i>) remain largely unknown. The present study analyzed the role of BRs in soybean response against <i>P. sojae</i> by comparative proteomic approaches. A total of 52,381 peptides were obtained by trypsin digestion of 9,680 proteins, among which 6,640 proteins were quantified, and 402 proteins were identified as differentially expressed proteins (DEPs). Further analysis revealed that DEPs were significantly involved in the lignin biosynthesis pathway. The expression of the majority of key enzymes involved in lignin biosynthesis was upregulated by BR-pretreatment and <i>P. sojae</i> infection, and lignin accumulation was faster in BR-pretreated soybeans than in untreated controls. Additionally, accumulation of lignin was consistent with these enzyme expressions levels and resistance phenotype. These findings advance the understanding of the role of BRs in the interaction between soybeans and <i>P. sojae</i>.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32302119</PMID><DateRevised><Year>2020</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><Volume>68</Volume><Issue>19</Issue><PubDate><Year>2020</Year><Month>May</Month><Day>13</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J Agric Food Chem</ISOAbbreviation></Journal><ArticleTitle>Comparative Proteomics Analysis Reveals That Lignin Biosynthesis Contributes to Brassinosteroid-Mediated Response to <i>Phytophthora sojae</i> in Soybeans.</ArticleTitle><Pagination><MedlinePgn>5496-5506</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.jafc.0c00848</ELocationID><Abstract><AbstractText>Brassinosteroids (BRs) are a group of steroid plant hormones regulating normal growth, development, and stress response in plants. However, the mechanisms by which BRs interfere with the resistance of soybean to <i>Phytophthora sojae</i> (<i>P. sojae</i>) remain largely unknown. The present study analyzed the role of BRs in soybean response against <i>P. sojae</i> by comparative proteomic approaches. A total of 52,381 peptides were obtained by trypsin digestion of 9,680 proteins, among which 6,640 proteins were quantified, and 402 proteins were identified as differentially expressed proteins (DEPs). Further analysis revealed that DEPs were significantly involved in the lignin biosynthesis pathway. The expression of the majority of key enzymes involved in lignin biosynthesis was upregulated by BR-pretreatment and <i>P. sojae</i> infection, and lignin accumulation was faster in BR-pretreated soybeans than in untreated controls. Additionally, accumulation of lignin was consistent with these enzyme expressions levels and resistance phenotype. These findings advance the understanding of the role of BRs in the interaction between soybeans and <i>P. sojae</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xinfang</ForeName><Initials>X</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-9125-7797</Identifier><AffiliationInfo><Affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Zisu</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Na</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Haitang</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Jinming</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xing</LastName><ForeName>Han</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Agric Food Chem</MedlineTA><NlmUniqueID>0374755</NlmUniqueID><ISSNLinking>0021-8561</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phytophthora sojae</Keyword><Keyword MajorTopicYN="N">brassinosteroids</Keyword><Keyword MajorTopicYN="N">lignin</Keyword><Keyword MajorTopicYN="N">proteomic analysis</Keyword><Keyword MajorTopicYN="N">soybean</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32302119</ArticleId><ArticleId IdType="doi">10.1021/acs.jafc.0c00848</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Xinfang" sort="Wang, Xinfang" uniqKey="Wang X" first="Xinfang" last="Wang">Xinfang Wang</name></noRegion><name sortKey="Guo, Na" sort="Guo, Na" uniqKey="Guo N" first="Na" last="Guo">Na Guo</name><name sortKey="Wang, Haitang" sort="Wang, Haitang" uniqKey="Wang H" first="Haitang" last="Wang">Haitang Wang</name><name sortKey="Xing, Han" sort="Xing, Han" uniqKey="Xing H" first="Han" last="Xing">Han Xing</name><name sortKey="Zhao, Jinming" sort="Zhao, Jinming" uniqKey="Zhao J" first="Jinming" last="Zhao">Jinming Zhao</name><name sortKey="Zhao, Zisu" sort="Zhao, Zisu" uniqKey="Zhao Z" first="Zisu" last="Zhao">Zisu Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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